MLK4 Antibody

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ProSci
Product Code: PSI-63-334
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-334-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase MLK4, Mixed lineage kinase 4, MLK4 {ECO:0000303|Ref1}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the p38 and JNK signaling cascades. Systematic mutational analyses of tyrosine kinases reveal that a minimum of 30% of colorectal cancer contain at least one mutation in the tyrosine kinases; sampling of colorect cancer cells suggest an important role of MLK4 in this process.
Background References:
  • Gregory,S.G., Nature 441 (7091), 315-321 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MLK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human MLK4.
NCBI Gene ID #:
84451
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase MLK4
NCBI Official Symbol:
MLK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
114 kDa
Protein Accession #:
Q5TCX8
Protein GI Number:
71153820
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q5TCX8
User NOte:
Optimal dilutions for each application to be determined by the researcher.