CSNK1E Antibody

ProSci
Product Code: PSI-62-885
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-885-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Casein kinase I isoform epsilon, CKI-epsilon, CKIe, CSNK1E
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CK1e is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. This protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. It has been shown to phosphorylate period, a circadian rhythm protein.
Background References:
  • Hino, S., et al., J. Biol. Chem. 278(16):14066-14073 (2003). Eide, E.J., et al., J. Biol. Chem. 277(19):17248-17254 (2002). Keesler, G.A., et al., Neuroreport 11(5):951-955 (2000). Cegielska, A., et al., J. Biol. Chem. 273(3):1357-1364 (1998). Kloss, B., et al., Cell 94(1):97-107 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CSNK1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human CSNK1E.
NCBI Gene ID #:
102800317, 1454
NCBI Official Name:
Casein kinase I isoform epsilon
NCBI Official Symbol:
CSNK1E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P49674
Protein GI Number:
1346369
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P49674
User NOte:
Optimal dilutions for each application to be determined by the researcher.