NKRF Antibody

Contact us
ProSci
Product Code: PSI-62-384
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-384-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of NKRF antibody in Jurkat cell line lysates (35ug/lane)
2 / 2
Formalin-fixed and paraffin-embedded human brain reacted with NKRF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of NKRF antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain reacted with NKRF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4, Transcription factor NRF, NKRF, ITBA4, NRF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.
Background References:
  • Ho,S.C., J. Immunol. 183 (1), 411-420 (2009)
  • Glanz,A., Immunol. Cell Biol. 86 (7), 622-630 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NKRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-233 amino acids from the Central region of human NKRF.
NCBI Gene ID #:
55922
NCBI Official Name:
NF-kappa-B-repressing factor
NCBI Official Symbol:
NKRF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
O15226
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O15226
User NOte:
Optimal dilutions for each application to be determined by the researcher.