ARFGAP1 Antibody

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ProSci
Product Code: PSI-61-363
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-363-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:8000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of ARFGAP1 Antibody against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right).
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Western Blot at 1:8000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ARFGAP1 Antibody against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right).

Documents

Further Information

Additional Names:
ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating protein, ARFGAP1, ARF1GAP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
Background References:
  • Huber, I., et al., Meth. Enzymol. 329, 307-316 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the C-terminal region of human ARFGAP1.
NCBI Gene ID #:
55738
NCBI Official Name:
ADP-ribosylation factor GTPase-activating protein 1
NCBI Official Symbol:
ARFGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q8N6T3
Protein GI Number:
27923731
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q8N6T3
User NOte:
Optimal dilutions for each application to be determined by the researcher.