PPP4C Antibody
| Code | Size | Price |
|---|
| PSI-57-248-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Serine/threonine-protein phosphatase 4 catalytic subunit, PP4C, Pp4, Protein phosphatase X, PP-X, PPP4C, PPP4, PPX
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
Background References:
- Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010)
- Mourtada-Maarabouni, M., et al. Leuk. Res. 33(11):1539-1551(2009)
- Kumar, R.A., et al. PLoS ONE(2), E4582 (2009) :
- Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus
Immunogen:
This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the Central region of human PPP4C.
NCBI Gene ID #:
5531
NCBI Official Name:
Serine/threonine-protein phosphatase 4 catalytic subunit
NCBI Official Symbol:
PPP4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P60510
Protein GI Number:
44888846
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P60510
User NOte:
Optimal dilutions for each application to be determined by the researcher.