PIP5KI gamma Antibody

ProSci
Product Code: PSI-63-359
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-359-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of hPIP5K1G-L652 in Hela cell line lysates (35ug/lane)
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Western blot analysis of PIP5K1C using PIP5K1G Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2).
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Western blot analysis of hPIP5K1G-L652 in Hela cell line lysates (35ug/lane)
Western blot analysis of PIP5K1C using PIP5K1G Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2).

Further Information

Additional Names:
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma, PIP5K1-gamma, PtdIns(4)P-5-kinase 1 gamma, Phosphatidylinositol 4-phosphate 5-kinase type I gamma, PIP5KIgamma, PIP5K1C, KIAA0589
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PIP5K1G is a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.
Background References:
  • Ling, K., et al., Nature 420(6911):89-93 (2002).
  • Di Paolo, G., et al., Nature 420(6911):85-89 (2002).
  • Ishihara, H., et al., J. Biol. Chem. 273(15):8741-8748 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIP5KI gamma (PIP5K1G) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-668 amino acids from the C-terminal region of human PIP5KI gamma (PIP5K1G).
NCBI Gene ID #:
23396
NCBI Official Name:
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
NCBI Official Symbol:
PIP5K1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
O60331
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O60331
User NOte:
Optimal dilutions for each application to be determined by the researcher.