SEC22B Antibody

ProSci
Product Code: PSI-57-454
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-454-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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SEC22B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela cell line lysates (35ug/lane).
SEC22B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Vesicle-trafficking protein SEC22b, ER-Golgi SNARE of 24 kDa, ERS-24, ERS24, SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, SEC22B, SEC22L1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins.
Background References:
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
  • Okumura, A.J., et al. J. Biol. Chem. 281(7):4495-4506(2006)
  • Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Mouse, Rat
Immunogen:
This SEC22B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the Central region of human SEC22B.
NCBI Gene ID #:
9554
NCBI Official Name:
Vesicle-trafficking protein SEC22b
NCBI Official Symbol:
SEC22B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
O75396
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O75396
User NOte:
Optimal dilutions for each application to be determined by the researcher.