JAM2 Antibody

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ProSci
Product Code: PSI-57-561
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-561-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from A431 cell line, using JAM2 Antibody at 1:1000 at each lane.
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JAM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from A431 cell line, using JAM2 Antibody at 1:1000 at each lane.
JAM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, JAM-2, Vascular endothelial junction-associated molecule, VE-JAM, CD322, JAM2, C21orf43, VEJAM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq].
Background References:
  • Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
  • Ueki, T., et al. Microvasc. Res. 75(2):269-278(2008)
  • Weber, C., et al. Nat. Rev. Immunol. 7(6):467-477(2007)
  • Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This JAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the N-terminal region of human JAM2.
NCBI Gene ID #:
58494
NCBI Official Name:
Junctional adhesion molecule B
NCBI Official Symbol:
JAM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P57087
Protein GI Number:
10720348
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P57087
User NOte:
Optimal dilutions for each application to be determined by the researcher.