SIRPB1 Antibody

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ProSci
Product Code: PSI-60-989
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-989-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SIRPB1 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 2
Western blot analysis in A375 cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SIRPB1 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in A375 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Signal-regulatory protein beta-1 isoform 3, SIRP-beta-1 isoform 3, SIRPB1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes (By similarity).
Background References:
  • Ota T., et al. Nat. Genet. 36:40-45(2004).
  • Bechtel S., et al. BMC Genomics 8:399-399(2007).
  • Deloukas P., et al. Nature 414:865-871(2001).
  • Hatherley D., et al. Mol. Cell 31:266-277(2008).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SIRPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-43 amino acids from the N-terminal region of human SIRPB1.
NCBI Gene ID #:
10326
NCBI Official Name:
Signal-regulatory protein beta-1 isoform 3
NCBI Official Symbol:
SIRPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q5TFQ8
Protein GI Number:
205829937
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q5TFQ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.