HOXB3 Antibody

ProSci
Product Code: PSI-56-515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-515-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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HOXB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in HL-60 cell line lysates (35ug/lane).
HOXB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Homeobox protein Hox-B3, Homeobox protein Hox-27, Homeobox protein Hox-2G, HOXB3, HOX2G
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML).
Background References:
  • Palakurthy, R.K., et al. Mol. Cell 36(2):219-230(2009)
  • Ferrai, C., et al. Mol. Biol. Cell 20(15):3543-3551(2009)
  • Chung, N., et al. Mol. Biol. Rep. 36(2):227-235(2009)
  • Speleman, F., et al. Leukemia 19(3):358-366(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HOXB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human HOXB3.
NCBI Gene ID #:
3213
NCBI Official Name:
Homeobox protein Hox-B3
NCBI Official Symbol:
HOXB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
P14651
Protein GI Number:
215274007
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P14651
User NOte:
Optimal dilutions for each application to be determined by the researcher.