ENTH Antibody

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ProSci
Product Code: PSI-62-499
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-499-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ENTH Antibody in HL-60 cell line lysates (35ug/lane)
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(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ENTH Antibody in HL-60 cell line lysates (35ug/lane)
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, Clint, Enthoprotin, Epsin-4, Epsin-related protein, EpsinR, CLINT1, ENTH, EPN4, EPNR, KIAA0171
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ENTH binds to membranes enriched in phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2). It May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. It stimulates clathrin assembly.
Background References:
  • Richards,M., et.al., J. Neural Transm. 115 (9), 1347-1354 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ENTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-249 amino acids from the Central region of human ENTH.
NCBI Gene ID #:
9685
NCBI Official Name:
Clathrin interactor 1
NCBI Official Symbol:
CLINT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q14677
Protein GI Number:
41016993
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q14677
User NOte:
Optimal dilutions for each application to be determined by the researcher.