RHOJ Antibody

ProSci
Product Code: PSI-56-029
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-029-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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RHOJ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).

RHOJ Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).

Further Information

Additional Names:
Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein, RHOJ, ARHJ, RASL7B, RHOI, TCL
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
  • Nishikimi, A., et al. FEBS Lett. 579(5):1039-1046(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RHOJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human RHOJ.
NCBI Gene ID #:
57381
NCBI Official Name:
Rho-related GTP-binding protein RhoJ
NCBI Official Symbol:
RHOJ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q9H4E5
Protein GI Number:
24418646
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H4E5
User NOte:
Optimal dilutions for each application to be determined by the researcher.