Guanylyl Cyclase alpha 1 Antibody

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ProSci
Product Code: PSI-62-679
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-679-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GUCY1A3 using GUCY1A3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GUCY1A3 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of GUCY1A3 using GUCY1A3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GUCY1A3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Guanylate cyclase soluble subunit alpha-3, GCS-alpha-3, GCS-alpha-1, Soluble guanylate cyclase large subunit, GUCY1A3, GUC1A3, GUCSA3, GUCY1A1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs.
Background References:
  • Saino, M., et al., Oncol. Rep. 12(1):47-52 (2004).
  • Zhou, Y., et al., Gene 245(2):319-328 (2000).
  • Papapetropoulos, A., et al., J. Cell. Physiol. 167(2):213-221 (1996).
  • Giuili, G., et al., Hum. Genet. 91(3):257-260 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Guanylyl Cyclase alpha 1 (GUCY1A3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the N-terminal region of human Guanylyl Cyclase alpha 1 (GUCY1A3).
NCBI Gene ID #:
2982
NCBI Official Name:
Guanylate cyclase soluble subunit alpha-3
NCBI Official Symbol:
GUCY1A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
Q02108
Protein GI Number:
7404351
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q02108
User NOte:
Optimal dilutions for each application to be determined by the researcher.