Glycerol kinase Antibody

ProSci
Product Code: PSI-62-677
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-677-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect GKP2 in Jurkat tissue lysate
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Antibody is used in Western blot to detect GKP2 in Jurkat tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Glycerol kinase 2, GK 2, Glycerokinase 2, ATP:glycerol 3-phosphotransferase 2, Glycerol kinase, testis specific 2, GK2, GKP2, GKTA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The human glycerol kinase gene family consists of at least 3 expressed loci. The GK1 locus on Xp21.3 is the site of mutations (deletions) causing glycerol kinase deficiency. It comprises 19 exons and is probably ancestral to several other genes which, because they are intronless, are suspected of having arisen by reverse transcriptase mediated events. These include 2 genes on chromosome 4. They are expressed as a single mRNA species in testis where expression is at a high level.
Background References:
  • Sargent, C.A., et al., Hum. Mol. Genet. 3(8):1317-1324 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Bovine
Immunogen:
This Glycerol kinase (GPK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human Glycerol kinase (GPK2).
NCBI Gene ID #:
2712
NCBI Official Name:
Glycerol kinase 2
NCBI Official Symbol:
GK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q14410
Protein GI Number:
212286188
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q14410
User NOte:
Optimal dilutions for each application to be determined by the researcher.