ProSci

CDH12 Antibody

Product Code:
 
PSI-57-950
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of anti-CDH12 Antibody in Jurkat cell line lysates (35ug/lane).
2 / 2
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH12 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of anti-CDH12 Antibody in Jurkat cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH12 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-57-950-400ul400ul£626.00
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Further Information

Additional Names:
Cadherin-12, Brain cadherin, BR-cadherin, Neural type cadherin 2, N-cadherin 2, CDH12
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
CDH12 is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.
Background References:
  • Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000)
  • Chalmers,I.J., Genomics 57 (1), 160-163 (1999)
  • Kremmidiotis,G., Genomics 49 (3), 467-471 (1998)
  • Selig,S., Proc. Natl. Acad. Sci. U.S.A. 94 (6), 2398-2403 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CDH12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human CDH12.
NCBI Gene ID #:
1010
NCBI Official Name:
Cadherin-12
NCBI Official Symbol:
CDH12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
P55289
Protein GI Number:
158937438
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P55289
User NOte:
Optimal dilutions for each application to be determined by the researcher.