ProSci

ECGF1 Antibody

Product Code:
 
PSI-62-801
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-ECGF1 Pab in HepG2 cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ECGF1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-ECGF1 Pab in HepG2 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ECGF1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-801-400ul400ul£626.00
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Further Information

Additional Names:
Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, PD-ECGF, TdRPase, TYMP, ECGF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
ECGF1 is an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in the gene encoding ECGF1 have been associated with mitochondrial neurogastrointestinal encephalomyopathy.
Background References:
  • Stenman,G.,Cytogenet. Cell Genet. 59 (1), 22-23 (1992)
  • Jinfeng,M.,Hepatogastroenterology 54 (78), 1635-1640 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ECGF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-345 amino acids from the Central region of human ECGF1.
NCBI Gene ID #:
1890
NCBI Official Name:
Thymidine phosphorylase
NCBI Official Symbol:
TYMP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P19971
Protein GI Number:
67477361
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P19971
User NOte:
Optimal dilutions for each application to be determined by the researcher.