ProSci

USO1 Antibody

Product Code:
 
PSI-57-431
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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USO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
USO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-431-400ul400ul£626.00
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Further Information

Additional Names:
General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
Background References:
  • Striegl, H., et al. PLoS ONE(2), E8991 (2010) :
  • Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
  • Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
  • Striegl, H., et al. PLoS ONE(2), E4656 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.
NCBI Gene ID #:
8615
NCBI Official Name:
General vesicular transport factor p115
NCBI Official Symbol:
USO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
108 kDa
Protein Accession #:
O60763
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O60763
User NOte:
Optimal dilutions for each application to be determined by the researcher.