ProSci

RYK Antibody

Product Code:
 
PSI-63-135
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-63-135-400ul400ul£626.00
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Further Information

Additional Names:
Tyrosine-protein kinase RYK, RYK, JTK5A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.
Background References:
  • Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002).
  • Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999).
  • Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996).
  • Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 561-591 amino acids from the C-terminal region of human RYK.
NCBI Gene ID #:
6259
NCBI Official Name:
Tyrosine-protein kinase RYK
NCBI Official Symbol:
RYK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
P34925
Protein GI Number:
1710811
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P34925
User NOte:
Optimal dilutions for each application to be determined by the researcher.