SEC63 Antibody

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ProSci
Product Code: PSI-56-867
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-867-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
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SEC63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in CEM cell line lysates (35ug/lane).
SEC63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Translocation protein SEC63 homolog, SEC63, SEC63L
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.
Background References:
  • Waanders, E., et al. Clin. Genet. 78(1):47-56(2010)
  • van Keimpema, L., et al. Liver Int. (2010) In press :
  • Waanders, E., et al. Histochem. Cell Biol. 129(3):301-310(2008)
  • You, K.T., et al. PLoS Biol.(5), E109 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SEC63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-168 amino acids from the N-terminal region of human SEC63.
NCBI Gene ID #:
11231
NCBI Official Name:
Translocation protein SEC63 homolog
NCBI Official Symbol:
SEC63
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
Q9UGP8
Protein GI Number:
18203500
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UGP8
User NOte:
Optimal dilutions for each application to be determined by the researcher.