PKC nu Antibody
| Code | Size | Price |
|---|
| PSI-62-599-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu, PRKD3, EPK2, PRKCN
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Background References:
- Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004).
- Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003).
- Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003).
- Bennasser, Y., et al., Virology 303(1):174-180 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.
NCBI Gene ID #:
23683
NCBI Official Name:
Serine/threonine-protein kinase D3
NCBI Official Symbol:
PRKD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
O94806
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O94806
User NOte:
Optimal dilutions for each application to be determined by the researcher.