KSR2 Antibody

ProSci
Product Code: PSI-55-850
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-850-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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KSR2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in 293,K562,HepG2,Hela cell line lysates (35ug/lane).
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KSR2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293,K562,HepG2,Hela cell line lysates (35ug/lane).

Further Information

Additional Names:
Kinase suppressor of Ras 2, hKSR2, KSR2
Application Note:
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Background References:
  • Bailey, S.D., et al. Diabetes Care (2010) In press :
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Liu, L., et al. Biochim. Biophys. Acta 1794(10):1485-1495(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KSR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-638 amino acids from the C-terminal region of human KSR2.
NCBI Gene ID #:
283455
NCBI Official Name:
Kinase suppressor of Ras 2
NCBI Official Symbol:
KSR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
108 kDa
Protein Accession #:
Q6VAB6
Protein GI Number:
148886599
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6VAB6
User NOte:
Optimal dilutions for each application to be determined by the researcher.