ERP29 Antibody

ProSci
Product Code: PSI-61-588
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-588-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ERP29 Antibody in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane)
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Western blot analysis of ERP29 using rabbit polyclonal ERP29 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).
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Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ERP29 Antibody in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane)
Western blot analysis of ERP29 using rabbit polyclonal ERP29 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 28, ERp28, Endoplasmic reticulum resident protein 31, ERp31, ERP29, C12orf8, ERP28
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Background References:
  • Bambang,I.F., et.al., Exp. Cell Res. 315 (11), 1964-1974 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ERP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-179 amino acids from the Central region of human ERP29.
NCBI Gene ID #:
10961
NCBI Official Name:
Endoplasmic reticulum resident protein 29
NCBI Official Symbol:
ERP29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P30040
Protein GI Number:
6015110
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P30040
User NOte:
Optimal dilutions for each application to be determined by the researcher.