LYN Antibody

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ProSci
Product Code: PSI-63-165
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-165-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-LYN Antibody in HL60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-LYN Antibody in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
Lyn is a protein tyrosine kinase which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis.
Background References:
  • Baran, C.P., et al., J. Biol. Chem. 278(40):38628-38636 (2003). Donato, N.J., et al., Blood 101(2):690-698 (2003). Santini, V., et al., Haematologica 87(12):1242-1247 (2002). Harashima, A., et al., Blood 100(13):4440-4445 (2002). Ren, X., et al., Cancer Biol. Ther. 1(6):703-708 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human LYN.
NCBI Gene ID #:
4067
NCBI Official Name:
Tyrosine-protein kinase Lyn
NCBI Official Symbol:
LYN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P07948
Protein GI Number:
125480
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P07948
User NOte:
Optimal dilutions for each application to be determined by the researcher.