ADIPOR1 Antibody

ProSci
Product Code: PSI-63-559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-559-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of ADIPOR1 Antibody in Y79 cell line lysates (35ug/lane)
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Flow cytometric analysis of widr cells using ADIPOR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ADIPOR1 Antibody in Y79 cell line lysates (35ug/lane)
Flow cytometric analysis of widr cells using ADIPOR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Adiponectin receptor protein 1, Progestin and adipoQ receptor family member I, ADIPOR1, PAQR1, TESBP1A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The adiponectin receptors, ADIPOR1 and ADIPOR2, serve as receptors for globular and full-length adiponectin and mediate increased AMPK and PPAR-alpha ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.
Background References:
  • Civitarese,A.E., et.al., Diabetologia 47 (5), 816-820 (2004)
  • Wang,H., et.al., Diabetes 53 (8), 2132-2136 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ADIPOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-331 amino acids from the C-terminal region of human ADIPOR1.
NCBI Gene ID #:
51094
NCBI Official Name:
Adiponectin receptor protein 1
NCBI Official Symbol:
ADIPOR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q96A54
Protein GI Number:
38372248
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q96A54
User NOte:
Optimal dilutions for each application to be determined by the researcher.