SFRP5 Antibody

ProSci
Product Code: PSI-62-574
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-574-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SFRP5 Antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of SFRP5 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina.
Background References:
  • Deloukas,P., et.al., Nature 429 (6990), 375-381 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 amino acids from the Central region of human SFRP5.
NCBI Gene ID #:
6425
NCBI Official Name:
Secreted frizzled-related protein 5
NCBI Official Symbol:
SFRP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q5T4F7
Protein GI Number:
150421670
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q5T4F7
User NOte:
Optimal dilutions for each application to be determined by the researcher.