DSTN Antibody

ProSci
Product Code: PSI-63-829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-829-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DSTN Antibody in MCF-7 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with DSTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of DSTN using rabbit polyclonal DSTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DSTN gene.

Western blot analysis of DSTN Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with DSTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of DSTN using rabbit polyclonal DSTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DSTN gene.

Further Information

Additional Names:
Destrin, Actin-depolymerizing factor, ADF, DSTN, ACTDP, DSN
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
DSTN belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This protein encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin).
Background References:
  • Estornes,Y., et.al., Int. J. Cancer 121 (10), 2162-2171 (2007)
  • Mannherz,H.G., et.al., J. Mol. Biol. 366 (3), 745-755 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This DSTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the Central region of human DSTN.
NCBI Gene ID #:
11034
NCBI Official Name:
Destrin
NCBI Official Symbol:
DSTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
P60981
Protein GI Number:
46577586
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P60981
User NOte:
Optimal dilutions for each application to be determined by the researcher.