KRT9 Antibody

ProSci
Product Code: PSI-62-813
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-813-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of in HL-60 cell line lysates (35ug/lane)
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KRT9 Antibody (Center K317) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.
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Western blot analysis of in HL-60 cell line lysates (35ug/lane)
KRT9 Antibody (Center K317) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.

Further Information

Additional Names:
Keratin, type I cytoskeletal 9, Cytokeratin-9, CK-9, Keratin-9, K9, KRT9
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
KRT9 is the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this protein cause epidermolytic palmoplantar keratoderma.
Background References:
  • Feng,W., Han,W. EurDermatol 18 (4), 387-390 (2008)
  • Zhao,J.J., Zhang,Z.H. Int. J. Dermatol. 47 (6), 634-637 (2008)
  • Torchard,D., Blanchet-Bardon,C. Nat. Genet.(1), 106-110 (1994)
  • Reis,A., Kuster,W. Hum. Genet. 90 (1-2), 113-116 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the Central region of human KRT9.
NCBI Gene ID #:
3857
NCBI Official Name:
Keratin, type I cytoskeletal 9
NCBI Official Symbol:
KRT9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
P35527
Protein GI Number:
239938886
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P35527
User NOte:
Optimal dilutions for each application to be determined by the researcher.