PITX1 Antibody

ProSci
Product Code: PSI-62-833
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-833-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)
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PITX1 Antibody (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of PITX1 using rabbit polyclonal PITX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.

Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)
PITX1 Antibody (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of PITX1 using rabbit polyclonal PITX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.

Further Information

Additional Names:
Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1, PITX1, BFT, PTX1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PITX1 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.
Background References:
  • Grimminger,P., Ling,F.C. Anticancer Res. 29 (4), 1281-1286 (2009)
  • Gurnett,C.A., Alaee,F. Am. J. Hum. Genet. 83 (5), 616-622 (2008)
  • Philippi,A., Tores,F. BMC Med. Genet. 8, 74 (2007)
  • Shang,J., Li,X. Genomics 40 (1), 108-113 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat, Xenopus, Zebrafish, Drosophila, C. Elegans
Immunogen:
This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human PITX1.
NCBI Gene ID #:
5307
NCBI Official Name:
Pituitary homeobox 1
NCBI Official Symbol:
PITX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P78337
Protein GI Number:
108935922
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P78337
User NOte:
Optimal dilutions for each application to be determined by the researcher.