ARL8 Antibody

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ProSci
Product Code: PSI-55-780
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-780-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
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ARL8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in CEM cell line lysates (35ug/lane).
ARL8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8, ARL5B, ARL8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.
Background References:
  • Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
  • Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
  • Sebald, E., et al. Gene 311, 147-151 (2003) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ARL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-154 amino acids from the C-terminal region of human ARL8.
NCBI Gene ID #:
221079
NCBI Official Name:
ADP-ribosylation factor-like protein 5B
NCBI Official Symbol:
ARL5B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q96KC2
Protein GI Number:
34922029
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q96KC2
User NOte:
Optimal dilutions for each application to be determined by the researcher.