HSPH1 Antibody

ProSci
Product Code: PSI-61-559
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-559-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HSPH1 Antibody in K562 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSPH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of HSPH1 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSPH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
HSPH1 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. This protein inhibits HSPA8/HSC70 ATPase and chaperone activities.
Background References:
  • Ishihara K., Yasuda K., Hatayama T.Biochim. Biophys. Acta 1444:138-142(1999)
  • Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y.,DNA Res. 3:321-329(1996)
  • The MGC Project Team Genome Res. 14:2121-2127(2004)
  • Miyazaki M., Nakatsura T., Yokomine K., Senju S., Monji M., Hosaka S.,Cancer Sci. 96:695-705(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Rat
Immunogen:
This HSPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-579 amino acids from the Central region of human HSPH1.
NCBI Gene ID #:
10808
NCBI Official Name:
Heat shock protein 105 kDa
NCBI Official Symbol:
HSPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q92598
Protein GI Number:
2495344
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q92598
User NOte:
Optimal dilutions for each application to be determined by the researcher.