PLOD1 Antibody

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ProSci
Product Code: PSI-56-571
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-571-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: U-2OS whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of lysates from A431, Hela, K562, MCF-7, U-87 MG cell line (from left to right), using PLOD1 Antibody at 1:1000 at each lane.
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Western blot analysis in U251 cell line lysates (35ug/lane).
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Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: U-2OS whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of lysates from A431, Hela, K562, MCF-7, U-87 MG cell line (from left to right), using PLOD1 Antibody at 1:1000 at each lane.
Western blot analysis in U251 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1, LH1, PLOD1, LLH, PLOD
Application Note:
For FACS starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000-1:2000
Background:
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Background References:
  • Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) :
  • Huang, Q.Y., et al. Bone 44(5):984-988(2009)
  • Yamada, Y., et al. Int. J. Mol. Med. 19(5):791-801(2007)
  • Tasker, P.N., et al. Osteoporos Int 17(7):1078-1085(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.
NCBI Gene ID #:
5351
NCBI Official Name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Official Symbol:
PLOD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q02809
Protein GI Number:
78099790
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q02809
User NOte:
Optimal dilutions for each application to be determined by the researcher.