SLC16A10 Antibody

ProSci
Product Code: PSI-56-938
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-938-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining SLC16A10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
2 / 5
Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysates Lane 2: CCRF-CEM whole cell lysates Lysates/proteins at 20 ug per lane.
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SLC16A10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antibody staining SLC16A10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysates Lane 2: CCRF-CEM whole cell lysates Lysates/proteins at 20 ug per lane.
SLC16A10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Monocarboxylate transporter 10, MCT 10, Aromatic amino acid transporter 1, Solute carrier family 16 member 10, T-type amino acid transporter 1, SLC16A10, MCT10, TAT1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SLC16A10 is a member of a family of plasma membrane amino acid transporters that mediate the Na(+)-independent transport of aromatic amino acids across the plasma membrane.
Background References:
  • Loubiere, L.S., et al. Placenta 31(4):295-304(2010)
  • Friesema, E.C., et al. Mol. Endocrinol. 22(6):1357-1369(2008)
  • Broer, S. Physiol. Rev. 88(1):249-286(2008)
  • Park, S.Y., et al. Arch. Pharm. Res. 28(4):421-432(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SLC16A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-329 amino acids from the C-terminal region of human SLC16A10.
NCBI Gene ID #:
117247
NCBI Official Name:
Monocarboxylate transporter 10
NCBI Official Symbol:
SLC16A10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q8TF71
Protein GI Number:
74751472
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8TF71
User NOte:
Optimal dilutions for each application to be determined by the researcher.