AFTPH Antibody

ProSci
Product Code: PSI-62-045
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-045-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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AFTPH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
AFTPH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Aftiphilin, AFTPH, AFTH
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
AFTPH may play a role in membrane trafficking.
Background References:
  • Lui-Roberts, W.W., et al. Mol. Biol. Cell 19(12):5072-5081(2008)
  • Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
  • Hirst, J., et al. Mol. Biol. Cell 16(5):2554-2565(2005)
  • Burman, J.L., et al. FEBS Lett. 579(10):2177-2184(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 844-871 amino acids from the C-terminal region of human AFTPH.
NCBI Gene ID #:
54812
NCBI Official Name:
Aftiphilin
NCBI Official Symbol:
AFTPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
Q6ULP2
Protein GI Number:
62286617
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6ULP2
User NOte:
Optimal dilutions for each application to be determined by the researcher.