SAR1A Antibody

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ProSci
Product Code: PSI-62-534
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-534-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody .AP6915c was diluted at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody .AP6915c was diluted at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
GTP-binding protein SAR1a, COPII-associated small GTPase, SAR1A, SAR1, SARA, SARA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
SAR1A is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity) and required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Background References:
  • Morgan,A.R., et.al., Am. J. Med. Genet.Neuropsychiatr. Genet. 144B (6), 762-770(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human SAR1A.
NCBI Gene ID #:
56681
NCBI Official Name:
GTP-binding protein SAR1a
NCBI Official Symbol:
SAR1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
Q9NR31
Protein GI Number:
14548013
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9NR31
User NOte:
Optimal dilutions for each application to be determined by the researcher.