KPNB1 Antibody

ProSci
Product Code: PSI-62-484
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-484-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of KPNB1 Antibody in Y79 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with KPNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of KPNB1 Antibody in Y79 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with KPNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97, KPNB1, NTF97
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
NTF97 is involved in nuclear protein import, either by associating itself with an adapter protein (for example, importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by acting autonomously as a nuclear transport receptor.
Background References:
  • Nordgard,S.H., et.al., Genes Chromosomes Cancer 47 (8), 680-696 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This KPNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-216 amino acids from the N-terminal region of human KPNB1.
NCBI Gene ID #:
3837
NCBI Official Name:
Importin subunit beta-1
NCBI Official Symbol:
KPNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q14974
Protein GI Number:
20981701
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q14974
User NOte:
Optimal dilutions for each application to be determined by the researcher.