DIAPH2 Antibody

ProSci
Product Code: PSI-63-930
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-930-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIAPH2, DIA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Yasuda, S., et al. Nature 428(6984):767-771(2004)
  • Gasman, S., et al. Nat. Cell Biol. 5(3):195-204(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 amino acids from the Central region of human DIAPH2.
NCBI Gene ID #:
1730
NCBI Official Name:
Protein diaphanous homolog 2
NCBI Official Symbol:
DIAPH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
126 kDa
Protein Accession #:
O60879
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O60879
User NOte:
Optimal dilutions for each application to be determined by the researcher.