SUMF1 Antibody

ProSci
Product Code: PSI-64-212
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-212-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of SUMF1 Antibody in Hela cell line lysates (35ug/lane)
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SUMF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of SUMF1 Antibody in Hela cell line lysates (35ug/lane)
SUMF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Sulfatase-modifying factor 1, 1899-, C-alpha-formylglycine-generating enzyme 1, SUMF1, FGE
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
SUMF1 encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine.
Background References:
  • Oshikawa, M., et al. Mol. Vis. 15, 482-494 (2009)
  • Fraldi, A., et al. Hum. Mol. Genet. 17(17):2610-2621(2008)
  • Hara, K., et al. Neurology 71(8):547-551(2008)
  • Yis, U., et al. Brain Dev. 30(5):374-377(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SUMF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the C-terminal region of human SUMF1.
NCBI Gene ID #:
285362
NCBI Official Name:
Sulfatase-modifying factor 1
NCBI Official Symbol:
SUMF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q8NBK3
Protein GI Number:
62298562
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8NBK3
User NOte:
Optimal dilutions for each application to be determined by the researcher.