EHD3 Antibody

ProSci
Product Code: PSI-62-929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-929-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EHD3 Antibody in CEM cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of EHD3 Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
EH domain-containing protein 3, PAST homolog 3, EHD3, EHD2, PAST3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
EHD3 plays a role in endocytic transport.
Background References:
  • Pohl U., Smith J.S.Genomics 63:255-262(2000)
  • George M., Ying G.BMC Cell Biol. 8:3-3(2007)
  • Sharma M., Naslavsky N.Traffic 9:995-1018(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the Central region of human EHD3.
NCBI Gene ID #:
30845
NCBI Official Name:
EH domain-containing protein 3
NCBI Official Symbol:
EHD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q9NZN3
Protein GI Number:
300669636
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9NZN3
User NOte:
Optimal dilutions for each application to be determined by the researcher.