Fascin Antibody

ProSci
Product Code: PSI-62-436
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-436-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, FSCN1, FAN1, HSN, SNL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.
Background References:
  • Qualtrough,D., Br. J. Cancer 101 (7), 1124-1129 (2009)
  • Ono,S., J. Biol. Chem. 272 (4), 2527-2533 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Fascin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the Central region of human Fascin.
NCBI Gene ID #:
6624
NCBI Official Name:
Fascin
NCBI Official Symbol:
FSCN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q16658
Protein GI Number:
2498357
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q16658
User NOte:
Optimal dilutions for each application to be determined by the researcher.