GPC6 Antibody

ProSci
Product Code: PSI-55-977
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-977-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GPC6 using rabbit polyclonal GPC6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.
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GPC6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of GPC6 using rabbit polyclonal GPC6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.
GPC6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Glypican-6, Secreted glypican-6, GPC6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq].
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Lau, C.S., et al. BrOphthalmol 94(3):357-362(2010)
  • Calboli, F.C., et al. PLoS ONE(7), E11504 (2010) :
  • Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GPC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-528 amino acids from the C-terminal region of human GPC6.
NCBI Gene ID #:
10082
NCBI Official Name:
Glypican-6
NCBI Official Symbol:
GPC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
Q9Y625
Protein GI Number:
9973298
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9Y625
User NOte:
Optimal dilutions for each application to be determined by the researcher.