KLC2 Antibody

Contact us
ProSci
Product Code: PSI-56-295
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-295-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of lysate from MCF-7 cell line, using KLC2 Antibody at 1:1000 at each lane.
2 / 3
KLC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
❮❯

Western blot analysis of lysate from MCF-7 cell line, using KLC2 Antibody at 1:1000 at each lane.
KLC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Kinesin light chain 2, KLC 2, KLC2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Kinesin is a molecular motor that generates ATP-dependent movement of vesicles and organelles along microtubules. Kinesin consists of 2 light chains, such as KLC2, and 2 heavy chains (see KIF5B; MIM 602809) in a 1:1 stoichiometric ratio (Rahman et al., 1998 [PubMed 9624122]).
Background References:
  • Trejo, H.E., et al. FASEB J. 24(2):374-382(2010)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the N-terminal region of human KLC2.
NCBI Gene ID #:
64837
NCBI Official Name:
Kinesin light chain 2
NCBI Official Symbol:
KLC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
Q9H0B6
Protein GI Number:
13878553
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H0B6
User NOte:
Optimal dilutions for each application to be determined by the researcher.