SRD5A3 Antibody

ProSci
Product Code: PSI-61-869
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-869-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).
2 / 3
SRD5A3 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).
SRD5A3 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Polyprenol reductase, 3-oxo-5-alpha-steroid 4-dehydrogenase 3, Steroid 5-alpha-reductase 2-like, Steroid 5-alpha-reductase 3, S5AR 3, SR type 3, SRD5A3, SRD5A2L
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
SRD5A3 converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Background References:
  • Uemura, M., et al. Cancer Sci. 99(1):81-86(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SRD5A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human SRD5A3.
NCBI Gene ID #:
79644
NCBI Official Name:
Polyprenol reductase
NCBI Official Symbol:
SRD5A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q9H8P0
Protein GI Number:
74733864
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H8P0
User NOte:
Optimal dilutions for each application to be determined by the researcher.