Calponin-3 Antibody

ProSci
Product Code: PSI-62-339
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-339-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of Calponin-3 antibody in NIH-3T3 cell line lysates (35ug/lane)
2 / 3
Calponin-3 Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of Calponin-3 antibody in NIH-3T3 cell line lysates (35ug/lane)
Calponin-3 Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Calponin-3, Calponin, acidic isoform, CNN3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Calponin-3 is a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. This protein is associated with the cytoskeleton but is not involved in contraction.
Background References:
  • Vinatzer,U., Clin. Cancer Res. 14 (20), 6426-6431 (2008)
  • Haag,J., Exp. Cell Res. 313 (16), 3386-3394 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This Calponin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human Calponin-3.
NCBI Gene ID #:
1266
NCBI Official Name:
Calponin-3
NCBI Official Symbol:
CNN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q15417
Protein GI Number:
6225157
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q15417
User NOte:
Optimal dilutions for each application to be determined by the researcher.