COG4 Antibody

ProSci
Product Code: PSI-62-906
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-906-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of COG4 Antibody in A2058 cell line lysates (35ug/lane)
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Flow cytometric analysis of jurkat cells using COG4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of COG4 Antibody in A2058 cell line lysates (35ug/lane)
Flow cytometric analysis of jurkat cells using COG4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.
Background References:
  • Whyte J.R., Munro S.Dev. Cell 1:527-537(2001)
  • Ota T., Suzuki Y., Nishikawa T.Nat. Genet. 36:40-45(2004)
  • Rikova K., Guo A., Zeng Q.Cell 131:1190-1203(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This COG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-785 amino acids from the C-terminal region of human COG4.
NCBI Gene ID #:
25839
NCBI Official Name:
Conserved oligomeric Golgi complex subunit 4
NCBI Official Symbol:
COG4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
89 kDa
Protein Accession #:
Q9H9E3
Protein GI Number:
311033464
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9H9E3
User NOte:
Optimal dilutions for each application to be determined by the researcher.