CCT3 Antibody

ProSci
Product Code: PSI-61-582
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-582-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CCT3 Antibody in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane)
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Western blot analysis of CCT3 using rabbit polyclonal CCT3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).
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Flow cytometric analysis of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CCT3 Antibody in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane)
Western blot analysis of CCT3 using rabbit polyclonal CCT3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).
Flow cytometric analysis of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
Background References:
  • Zebol,J.R., Int. J. Biochem. Cell Biol. 41 (4), 822-827 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat, Xenopus
Immunogen:
This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the Central region of human CCT3.
NCBI Gene ID #:
7203
NCBI Official Name:
T-complex protein 1 subunit gamma
NCBI Official Symbol:
CCT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P49368
Protein GI Number:
66774185
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P49368
User NOte:
Optimal dilutions for each application to be determined by the researcher.