RSBN1 Antibody

ProSci
Product Code: PSI-55-902
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-902-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Antibody staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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RSBN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antibody staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
RSBN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Round spermatid basic protein 1, RSBN1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Prahalad, S., et al. Arthritis Rheum. 60(7):2124-2130(2009)
  • Cooper, J.D., et al. Nat. Genet. 40(12):1399-1401(2008)
  • Raychaudhuri, S., et al. Nat. Genet. 40(10):1216-1223(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
NCBI Gene ID #:
54665
NCBI Official Name:
Round spermatid basic protein 1
NCBI Official Symbol:
RSBN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
Q5VWQ0
Protein GI Number:
257050986
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q5VWQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.