MOB4A Antibody

Contact us
ProSci
Product Code: PSI-62-608
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-608-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.
2 / 4
Western blot analysis in Daudi,K562 cell line lysates (35ug/lane).
3 / 4
4 / 4
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.
Western blot analysis in Daudi,K562 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1B, MOB4A, MOBKL1A
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.
Background References:
  • Bichsel,S.J.,J. Biol. Chem. 279 (34), 35228-35235 (2004) Devroe,E.,J. Biol. Chem. 279 (23), 24444-24451 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
NCBI Gene ID #:
92597
NCBI Official Name:
MOB kinase activator 1B
NCBI Official Symbol:
MOB1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
Q7L9L4
Protein GI Number:
56749324
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q7L9L4
User NOte:
Optimal dilutions for each application to be determined by the researcher.