ProSci

PLA2G6 Antibody

Product Code:
 
PSI-64-196
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PLA2G6 Antibody in HepG2 cell line lysates (35ug/lane)
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PLA2G6 Antibody IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of PLA2G6 Antibody in HepG2 cell line lysates (35ug/lane)
PLA2G6 Antibody IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-64-196-400ul400ul£626.00
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Further Information

Additional Names:
85/88 kDa calcium-independent phospholipase A2, CaI-PLA2, Group VI phospholipase A2, GVI PLA2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, iPLA2-beta, Patatin-like phospholipase domain-containing protein 9, PNPLA9, PLA2G6, PLPLA9
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.
Background References:
  • Ayilavarapu, S., et al. J. Immunol. 184(3):1507-1515(2010) Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010) Tan, E.K., et al. Ann. Neurol. 67 (1), 148 (2010) Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLA2G6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-580 amino acids from the Central region of human PLA2G6.
NCBI Gene ID #:
8398
NCBI Official Name:
85/88 kDa calcium-independent phospholipase A2
NCBI Official Symbol:
PLA2G6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
O60733
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O60733
User NOte:
Optimal dilutions for each application to be determined by the researcher.