ProSci

VPS52 Antibody

Product Code:
 
PSI-55-511
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
2 / 4
VPS52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Confocal immunofluorescent analysis of VPS52 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
VPS52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of VPS52 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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PSI-55-511-400ul400ul£626.00
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Further Information

Additional Names:
Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein, VPS52, SACM2L
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
Background References:
  • Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009)Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009) Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008) Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005) Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-637 amino acids from the C-terminal region of human VPS52.
NCBI Gene ID #:
6293
NCBI Official Name:
Vacuolar protein sorting-associated protein 52 homolog
NCBI Official Symbol:
VPS52
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82 kDa
Protein Accession #:
Q8N1B4
Protein GI Number:
74750887
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8N1B4
User NOte:
Optimal dilutions for each application to be determined by the researcher.