DPT Antibody

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ProSci
Product Code: PSI-62-945
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-945-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DPT antibody in HL60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain with DPT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Immunofluorescence analysis of DPT Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
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Western blot analysis of DPT antibody in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain with DPT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescence analysis of DPT Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Documents

Further Information

Additional Names:
Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
DPT is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. This protein is found in various tissues and many of its tyrosine residues are sulphated. The protein is postulated to modify the behavior of TGF-beta through interaction with decorin.
Background References:
  • Cheung,C.L., Chan,B.Y. Hum. Mol. Genet. 18 (4), 679-687 (2009)
  • Pochampally,R.R., Ylostalo,J. J. Bone Miner. Res. 22 (9), 1338-1349 (2007)
  • Lunetta,K.L., D'Agostino,R.B. Sr. BMC Med. Genet.SUPPL 1, S13 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This DPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-128 amino acids from the Central region of human DPT.
NCBI Gene ID #:
1805
NCBI Official Name:
Dermatopontin
NCBI Official Symbol:
DPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q07507
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q07507
User NOte:
Optimal dilutions for each application to be determined by the researcher.